What AOD-9604 is
AOD-9604 is a development code for a 16-residue synthetic peptide, Tyr-Leu-Arg-Ile-Val-Gln-Cys-Arg-Ser-Val-Glu-Gly-Ser-Cys-Gly-Phe (YLRIVQCRSVEGSCGF). Its two cysteines, at positions 7 and 14, are joined by a disulfide bond that closes the central part of the chain into a loop, and the C-terminus is a free acid. Every residue is a standard L-amino acid.
The sequence is taken from the C-terminal tail of human growth hormone: it corresponds to hGH residues 177 to 191 with an extra tyrosine added at the N-terminus, which is the same as the 176 to 191 stretch with its first phenylalanine swapped for tyrosine. That detail matters because the unmodified hGH(176–191) fragment begins with phenylalanine and is about 16 Da lighter, so a listing that says only “hGH fragment 176–191” does not by itself identify AOD-9604.
AOD-9604 5mg specification
| Catalog name | AOD-9604 |
|---|---|
| Sequence | H-Tyr-Leu-Arg-Ile-Val-Gln-Cys-Arg-Ser-Val-Glu-Gly-Ser-Cys-Gly-Phe-OH (disulfide Cys7–Cys14) |
| Molecular formula | C78H123N23O23S2 (disulfide form) |
| Average molecular mass | approx. 1815.1 g/mol |
| Amount per vial | 5 mg |
| Form | Lyophilized powder, sealed vial |
| HPLC purity | ≥98% |
| Shelf life | 36 months in the sealed vial |
How AOD-9604 is analyzed
With two arginines and a glutamate balanced against leucine, isoleucine, two valines and the two aromatic end residues, AOD-9604 retains moderately on C18 and elutes mid-gradient under standard water/acetonitrile/TFA conditions. A single tyrosine and a single phenylalanine give only weak 280 nm absorbance, so purity is integrated near 214 nm. Electrospray usually shows the doubly and triply protonated ions, close to m/z 908.6 and 606.0 for the cyclized peptide.
The disulfide creates impurity classes a linear peptide does not have: the reduced dithiol form, 2 Da heavier and typically eluting at a different time, and disulfide-linked dimers near twice the mass. A mass about 16 Da low points to the phenylalanine-starting fragment rather than this sequence. Reverse-phase HPLC on this product is carried out independently and its purity result is posted on this page; the vial has no lot or batch marking, so its crimp and cap color connects it to the posted AOD-9604 result.
Research contexts for AOD-9604
- Sequence-identity work distinguishing Tyr-started AOD-9604 from the Phe-started hGH(176–191) fragment
- Disulfide mapping and oxidized/reduced form separation by LC-MS
- Structural studies of C-terminal growth hormone fragments by circular dichroism or NMR
- Protease stability comparisons of looped versus linearized fragment peptides in cell-free systems
This AOD-9604 is a reference peptide for bench and instrument work in vitro only, with no intended use in people or animals.
AOD-9604 questions
Is AOD-9604 the same as hGH fragment 176–191?
Not quite. AOD-9604 starts with tyrosine, while the native 176–191 fragment starts with phenylalanine, and the two differ by one hydroxyl group, roughly 16 Da.
What mass should the spectrum show?
About 1815.1 for the disulfide-bonded peptide. A value near 1817 indicates the reduced form, and a peak near 3628 suggests a disulfide-linked dimer.
Why does the formula state the disulfide form?
Closing the Cys7–Cys14 bridge removes two hydrogen atoms, so the cyclized and reduced forms have different formulas and masses. The specification describes the bridged molecule.
Buying AOD-9604 5mg in the USA
AOD-9604 5mg is shipped from within the US and tracked to the door, with free shipping on orders over $100. Bulk pricing is offered to groups ordering repeatedly for sequence or disulfide work. AOD-9604 is available only to research institutions, licensed researchers, universities and laboratory R&D purchasers. For the complete sequence and numbering reference, see What Is AOD-9604? Sequence, Identity and Analytical Profile.
AOD-9604 5mg is a research chemical for in-vitro laboratory use. Human and veterinary use are excluded, and this listing contains no preparation or dosing guidance.
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