What MOTS-c is
MOTS-c is a linear 16-residue peptide, Met-Arg-Trp-Gln-Glu-Met-Gly-Tyr-Ile-Phe-Tyr-Pro-Arg-Lys-Leu-Arg, or MRWQEMGYIFYPRKLR in one-letter code. Every residue is a standard L-amino acid, the N-terminus is a free amine and the C-terminus a free acid, with no capping or modification. There is no cysteine in the sequence, so the synthetic material is a single open chain with no disulfide to form or verify.
The name is an abbreviation for mitochondrial open reading frame of the 12S rRNA-c. The sequence is encoded by a short reading frame inside the mitochondrial MT-RNR1 gene rather than in nuclear DNA, which places it among the mitochondrially derived peptides, a small group that also includes humanin from the neighboring 16S rRNA gene. The name records a genomic location, not a property, and at sixteen residues there is ample room for a truncated or substituted chain to be sold under the same label, so the printed sequence is the real identifier.
MOTS-c 10mg specification
| Catalog name | MOTS-c |
|---|---|
| Sequence | H-MRWQEMGYIFYPRKLR-OH |
| Molecular formula | C101H152N28O22S2 |
| Average mass | approx. 2174.6 g/mol |
| CAS number | 1627580-64-6 |
| Amount per vial | 10 mg |
| Form | Lyophilized powder, sealed vial |
| HPLC purity | ≥98% |
| Storage specification | −20 °C, dry |
| Shelf life | 36 months (sealed vial) |
How MOTS-c is analyzed
Three arginines and a lysine against a single glutamate give MOTS-c a clear net positive charge in a TFA mobile phase, while tryptophan, two tyrosines, phenylalanine, isoleucine, leucine and two methionines provide a substantial hydrophobic cluster. The result is solid retention on C18 and elution in the middle of a water/acetonitrile gradient. With one Trp and two Tyr it reads well at 280 nm, and comparing that trace with the 214 nm trace helps separate sequence-related impurities from non-aromatic contaminants. The two sulfur atoms in the formula come from the methionines, and each can oxidize to the sulfoxide, giving +16 and +32 Da species that usually elute just before the main peak; tryptophan oxidation adds mass in the same 16 Da steps. Electrospray produces a series of charge states whose deconvoluted value should sit near 2174.6.
MOTS-c has its reverse-phase HPLC result published on this page. Since no lot or batch number appears on the vial, the crimp and cap color serves as the link between vial and published test.
Research contexts for MOTS-c
- Sequence and nomenclature studies of mitochondrially encoded peptides
- Cell-culture assays comparing MOTS-c with other mitochondrially derived peptides such as humanin
- Methionine and tryptophan oxidation profiling as an analytical stress model
- Charge-state deconvolution and counter-ion quantification for mid-sized basic peptides
MOTS-c is a reference material for in-vitro laboratory research, not for use in people or animals.
MOTS-c questions
What does the name MOTS-c stand for?
Mitochondrial open reading frame of the 12S rRNA-c. It describes where the coding sequence sits in the mitochondrial genome.
What do small peaks just ahead of the MOTS-c main peak usually mean?
Most often methionine sulfoxide forms. They are slightly more polar than the parent and appear at +16 Da, or +32 Da when both methionines are oxidized.
Does MOTS-c contain a disulfide bond?
No. The sequence has no cysteine, so identity depends only on the linear sequence and its mass.
Buying MOTS-c 10mg in the USA
MOTS-c goes out from our US base with a tracking number attached, and shipping costs nothing on orders exceeding $100. Bulk pricing suits labs running MOTS-c across several assay rounds. Accounts are open only to research institutions, licensed researchers, universities and laboratory R&D purchasers. Read What Is MOTS-c? Sequence, Identity and Analytical Profile for the full identity reference.
MOTS-c 10mg is sold strictly for in-vitro laboratory research and is not for human or veterinary use.
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