GLP-1/GIP – 10mg

What GLP-1/GIP 10mg is

This listing supplies 10 mg of the compound catalogued as GLP-1/GIP, the dual receptor agonist peptide in our receptor-named group. The name follows receptor pharmacology convention: the molecule is characterized in vitro against both the GLP-1 receptor and the GIP receptor, two related class B GPCRs. How that convention works, and why the catalog uses it, is covered in our GLP-1, GIP and glucagon nomenclature reference.

The peptide is 39 amino acids long and amidated at the C-terminus. Non-coded Aib residues sit at the second and thirteenth positions, the chain carries two lysines of which only one is modified, and that modified lysine bears a 20-carbon diacid tethered by a γ-glutamyl unit and two short oxyethylene amino acid spacers. The free lysine and the amidated terminus distinguish it chemically from the single-receptor GLP-1 product, which ends in a free acid.

GLP-1/GIP 10mg specification

How GLP-1/GIP 10mg is analyzed

At close to 4.8 kDa with a long lipid tail, this is among the more hydrophobic items in the catalog, and it needs a gradient that climbs to high organic strength before it elutes. Mobile-phase additives matter here: trifluoroacetic acid gives sharp peaks for UV work but suppresses electrospray signal, so identity runs often switch to formic acid at some cost in peak shape. The unmodified lysine and N-terminal tyrosine give the molecule several basic and aromatic handles, and its 4+ and 5+ ions are expected near m/z 1204 and 964.

Isomeric impurities are the hard part: aspartate isomerization and epimerization at a single residue leave the mass unchanged and can only be seen chromatographically. Oxidized tryptophan (+16 Da), glutamine deamidation and species acylated at the wrong lysine are the other classes to look for, the last one sharing the exact mass of the target. This product’s own reverse-phase HPLC purity, from independent testing, is displayed on this page; vials are printed without lot or batch codes, so matching to that result relies on crimp and cap color.

Research contexts for GLP-1/GIP 10mg

• Concentration-response series in cell-based assays run side by side on GLP-1 receptor and GIP receptor lines
• Radioligand or fluorescent competition binding on isolated receptor membrane preparations
• Positional-isomer method development, separating acylation at one lysine from the other
• Comparative LC-MS of the single, dual and triple receptor agonist peptides

The compound is sold for cell-free and cell-culture laboratory studies only; no application in humans or animals is intended.

GLP-1/GIP 10mg questions

Why would two impurities have the same mass as the main peak?

Aspartate isomers and positional isomers of the lipid chain have identical formulas, so a mass spectrum cannot flag them. Only retention differences reveal them.

Is the 10 mg product the same compound as the 5 mg one?

Yes. The formula C225H348N48O68 and average mass of about 4813.5 g/mol are the same; the vial simply holds twice the stated amount.

Why does formic acid change the chromatogram?

Formic acid is a weaker ion-pairing agent than trifluoroacetic acid, so the peptide retains a little less and peaks broaden, while mass-spectrometric sensitivity improves.