GLP-1 – 10mg

What GLP-1 10mg contains

The catalog item called GLP-1 is a lipidated synthetic peptide that in-vitro receptor pharmacology groups as a single receptor agonist: it is characterized against one target, the GLP-1 receptor, which belongs to the class B family of G-protein-coupled receptors. Labeling by receptor rather than by any other name is deliberate, and our guide to GLP-1, GIP and glucagon nomenclature sets out why. This listing is the larger 10 mg fill of the same compound offered in 5 mg vials.

The backbone runs to 31 amino acids ending in a free carboxylic acid. Two features set it apart from a plain linear peptide. An α-aminoisobutyric acid, the gem-dimethyl analog of alanine, replaces a coded amino acid near the N-terminus. The only lysine carries an 18-carbon dicarboxylic acid, connected through a γ-glutamic acid and two short ethylene-glycol-based amino acid units. Those additions account for the formula below.

GLP-1 10mg specification

How GLP-1 10mg is analyzed

Lipidated peptides behave differently from ordinary sequences on a column. The long diacid chain promotes self-association, so peak tailing, carryover from one run to the next and concentration-dependent peak shape are all more common than with an unmodified 31-mer. Butyl or octyl phases with 300 Å pores, or a C18 run warm, are the usual ways to keep the main peak narrow. At this size, average and monoisotopic masses differ by a couple of daltons, so a mass report should say which it quotes; the 4+ and 5+ ions fall close to m/z 1029 and 824.

Typical related substances include the peptide lacking its lipid side chain, which elutes much earlier, oxidized tryptophan, deamidated glutamine and a D-histidine epimer at the N-terminal residue, which can sit just beside the main peak. Every product in the catalog is run independently by reverse-phase HPLC. The GLP-1 figure is shown here; with no lot or batch code printed on any vial, matching the crimp and cap color to the published chromatogram is how a 10 mg vial is traced.

Research contexts for GLP-1 10mg

• Concentration-response series in cell-based GLP-1 receptor assays, where the larger fill supports replicate plates
• Competition binding against labeled ligands on isolated membrane preparations
• Aggregation and self-association studies of lipopeptides by size-exclusion chromatography or light scattering
• Reference material for comparing single, dual and triple receptor agonist peptides by LC-MS

Intended strictly for bench and instrument work in vitro; this reagent has no use in people or animals.

GLP-1 10mg questions

Is the 10 mg vial a different compound from the 5 mg vial?

No. Formula, mass and CAS number are identical; only the stated amount in the vial differs.

Does 10 mg on the label mean 10 mg of peptide?

A labeled amount and a net peptide content are separate figures, because counter-ions and residual water also have mass. Our article on what the mg on a peptide vial means explains the distinction.

What is α-aminoisobutyric acid doing in the sequence?

Aib is a non-coded residue with a gem-dimethyl alpha carbon. It constrains the local backbone conformation and adds 14 Da relative to an alanine at the same position, which is one reason the formula differs from a sequence built only from coded residues.