BPC-157 5mg

What BPC-157 is

BPC-157 is a linear synthetic pentadecapeptide with the sequence Gly-Glu-Pro-Pro-Pro-Gly-Lys-Pro-Ala-Asp-Asp-Ala-Gly-Leu-Val, or GEPPPGKPADDAGLV in one-letter code. All fifteen residues are ordinary L-amino acids, the chain contains no cysteine and so no disulfide, and neither terminus is capped. The letters come from a phrase in the early literature, and the number is a laboratory designation with no structural meaning.

Two features set it apart from its catalog neighbors. Four of its fifteen positions are proline, three of them in an unbroken run at positions 3 to 5, and its only acidic cluster is an Asp-Asp pair at positions 10 and 11. BPC-157 is also sold here as one component of a three-peptide blend, which is a separate product with its own total mass and analysis.

BPC-157 5mg specification

How BPC-157 is analyzed

The proline run is what makes BPC-157 unusual on a reverse-phase column. Each Xaa-Pro bond can adopt either a cis or a trans geometry, and the two forms interconvert over seconds to minutes, roughly the same timescale as the separation itself. The main band is therefore often wide, sometimes shouldered, even in clean material; a hotter column speeds the exchange and narrows it, whereas a genuine impurity keeps its own peak. The sequence has no aromatic side chains, so detection relies on the amide backbone near 214 nm, and electrospray MS should show a singly protonated ion close to m/z 1420 with a doubly charged ion near 710.

The Asp-Asp pair is the main chemical liability. Aspartate can cyclize to a succinimide, losing 18 Da, and reopen as isoaspartate, which has the same mass as the parent and tends to hide inside the already broad main band. The independent reverse-phase HPLC result for BPC-157 5mg is shown on this product page; no number appears on the vial, so matching it to that run relies on the crimp and cap color.

Research contexts for BPC-157

• Chromatographic studies of proline cis-trans isomerism and temperature-dependent peak shape
• Succinimide and isoaspartate formation in sequences carrying an Asp-Asp motif
• Signaling assays in endothelial and fibroblast cell cultures
• Enzymatic-digestion studies of proline-rich stretches that resist common proteases

Every BPC-157 vial we ship is a bench reference for cell-free, cell-culture and chromatographic study, and none is meant to enter a person or an animal.

BPC-157 questions

Is a broad BPC-157 peak a sign of low purity?

Not on its own. Slow cis-trans exchange at the three consecutive prolines widens the band in clean material, so an orthogonal check, such as a hotter column or an LC-MS scan across the peak, is what separates conformers from a real contaminant.

Why can’t a mass spectrum alone rule out isoaspartate in BPC-157?

Isoaspartate is an isomer of aspartate with an identical mass, so the intact reading stays near 1419.5 either way. It has to be resolved chromatographically or identified with more specialized techniques.

How does this vial differ from the BPC-157 + TB500 + MGF blend?

This vial holds 5 mg of the single pentadecapeptide; in the 18.5 mg blend, BPC-157 is one of three components analyzed together as a mixture.