Melanotan 2 10mg

What Melanotan 2 is

Melanotan 2 (MT-2, MT-II) is a cyclic heptapeptide with the structure Ac-Nle-cyclo[Asp-His-D-Phe-Arg-Trp-Lys]-NH2. A lactam bond between the side-chain carboxyl of aspartic acid and the side-chain amine of lysine closes six of the seven residues into a ring, leaving an acetylated norleucine outside it as a short tail. The lysine that completes the ring ends in a carboxamide.

Structurally it is a truncated, cyclized analog of alpha-MSH positions 4 to 10, carrying four changes: Nle4 for Met, Asp5 for Glu, D-Phe7, and Lys10 for Gly. Public databases therefore list it under a descriptive name along the lines of Ac-[Nle4, Asp5, D-Phe7, Lys10]-cyclo-alpha-MSH(4-10) amide. It is not a shortened Melanotan 1, which is linear and thirteen residues long. Its nearest neighbor is PT-141, which shares the same ring and tail and differs only by ending in a free acid instead of an amide.

Melanotan 2 10mg specification

How Melanotan 2 is analyzed

The ring restricts the conformations MT-2 can adopt, and constrained peptides of this kind usually give a sharper, more symmetrical reverse-phase peak than a flexible chain of similar mass. Norleucine, D-phenylalanine and tryptophan supply the hydrophobic surface that keeps it well retained on C18, while arginine and histidine are protonated in an acidic mobile phase. Tryptophan absorbs strongly at 280 nm, though the purity integration is taken near 214 nm. Two mass checks are specific to this molecule. The observed mass should agree with the amide at about 1024.2 rather than the free acid at about 1025.2, which calls for a figure reported to one decimal place. A signal about 18 Da high points to linear precursor that never closed its lactam, since ring formation releases one water molecule.

This page carries the published reverse-phase HPLC result for Melanotan 2. Instead of lot or batch numbers, each vial is paired with that test through its crimp and cap color.

Research contexts for Melanotan 2

• Competitive binding studies across the five melanocortin receptor subtypes (MC1R to MC5R) in membrane preparations
• Second-messenger (cAMP) assays in recombinant cell lines, as a cyclic reference ligand for melanocortin receptor pharmacology
• Conformational studies of lactam-bridged macrocycles by NMR or circular dichroism
• Analytical method development for separating C-terminal amide and free-acid forms

We supply Melanotan 2 for in-vitro and analytical laboratory studies only, never for administration to a person or an animal.

Melanotan 2 questions

How can Melanotan 2 be told apart from PT-141?

Only by the C-terminus. MT-2 is the amide and PT-141 the free acid, a difference of about 1 Da, so a mass stated to one decimal place and read against the correct calculated value is the reliable check. Retention time by itself is not enough.

What does an extra 18 Da signal indicate for MT-2?

It is consistent with the uncyclized linear precursor, which retains the water molecule lost when the Asp-Lys lactam forms.

Is Melanotan 2 an updated form of Melanotan 1?

No. Despite the numbering, it is a different macrocyclic design with seven residues rather than thirteen and roughly 620 Da less mass.