Gonadorelin 10mg

What Gonadorelin is

Gonadorelin is the nonproprietary name for the synthetic form of native GnRH (also written LHRH), a decapeptide with the sequence pGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2. Both ends are blocked. The first residue is pyroglutamate, a glutamine whose side chain has cyclized onto its own alpha-amine to form a five-membered lactam, and the last is a glycinamide. The molecule therefore has neither a free N-terminal amine nor a free C-terminal carboxyl.

In catalog usage gonadorelin, GnRH and LHRH all mean this same unmodified ten-residue sequence. Substituted analogs, typically carrying a D-amino acid at position 6 or an altered C-terminus, are different compounds with different masses, and an analysis of one says nothing about the others. Identifying the material by sequence and mass, rather than by the name on the label, is the only dependable approach in this family.

Gonadorelin 10mg specification

How Gonadorelin is analyzed

Because the pyroglutamate leaves no free alpha-amine, Edman degradation cannot begin on this peptide, so identity rests on mass spectrometry and tandem MS fragmentation rather than N-terminal sequencing. Tryptophan and tyrosine make it a rare case where a dual-wavelength trace is genuinely useful: an impurity visible at 214 nm but absent at 280 nm lacks an aromatic residue and is unlikely to be a modified form of the target. Tryptophan is also the main oxidation risk, with oxidized species appearing at +16 Da and often just ahead of the main peak. A C-terminal free acid, a known synthesis by-product, reads about 1 Da high, and deletion sequences from incomplete couplings make up the remaining impurity class. Arginine and histidine bind counter-ion readily, so net peptide content sits noticeably below chromatographic purity.

Gonadorelin’s own reverse-phase HPLC result is posted on this page. Vials are not lot- or batch-coded; the crimp and cap color identifies which published test a given vial belongs to.

Research contexts for Gonadorelin

• GnRH receptor (GNRHR) binding and displacement assays in transfected cell membranes
• Reference ligand for comparing position-6 and C-terminal substituted decapeptide analogs in vitro
• Tandem MS sequencing method development for N-terminally blocked peptides
• Tryptophan oxidation and C-terminal deamidation studies as analytical stress models

Gonadorelin is offered as a cell-culture and cell-free research reagent, and none of it is intended for people or animals.

Gonadorelin questions

Why can Gonadorelin not be sequenced by Edman chemistry?

Edman degradation needs a free N-terminal amine to react with, and the cyclized pyroglutamate at position 1 has none. Mass spectrometry is used for sequence confirmation instead.

What mass should the spectrum show for Gonadorelin?

About 1182.3 g/mol for the free base. A salt form reads heavier on the balance, and a free-acid C-terminus appears roughly 1 Da above the amide.

Are Gonadorelin, GnRH and LHRH one and the same molecule?

In catalog terms, yes: all three names describe the native decapeptide. Analogs with substituted residues are separate compounds.