Gonadorelin is the common name for synthetic gonadotropin-releasing hormone. This article is an identity and analytical reference for the material sold under that name as a laboratory reference standard: sequence, terminal chemistry, expected mass and how the compound behaves under analysis. It makes no claim about what the compound does in any organism, and it contains no preparation, handling or dosing guidance.
The sequence
Gonadorelin is a decapeptide: pGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2. The free-base average mass is approximately 1,182 g/mol.
Two features of that sequence do most of the analytical work, and both are terminal.
Why the terminal chemistry matters more than usual
The N-terminus is pyroglutamate. The first residue is a cyclised glutamine, not a free glutamate, and it leaves no free alpha-amino group. That has a direct practical consequence: classical Edman degradation sequencing cannot start on this peptide, because Edman chemistry requires a free N-terminal amine. Identity confirmation therefore leans on mass spectrometry rather than on N-terminal sequencing, and a supplier offering “sequence confirmation” should be asked what method produced it.
The C-terminus is an amide, not a free acid. A C-terminal amide is 1 Da lighter than the corresponding free acid. On a ten-residue peptide that is a small difference against a large number, and it is exactly the kind of difference that gets lost if a certificate reports a rounded mass. If the observed mass sits a unit high, the material may be the free-acid form — a related but different compound, and a known synthesis by-product.
Detection is straightforward, for once
Unlike the short bioregulator peptides, Gonadorelin contains both tryptophan and tyrosine. Those are strong ultraviolet chromophores, so the compound can be read at 280 nm as well as at the usual 214 nm peptide-bond wavelength. A dual-wavelength trace is genuinely informative here: an impurity peak that appears at 214 but not at 280 lacks an aromatic residue and is therefore unlikely to be a truncated or modified form of the target sequence.
Impurities worth looking for on the trace
Tryptophan is the most oxidation-prone residue in the standard set, and an oxidised tryptophan adds 16 Da. On a decapeptide that is a resolvable shift on a competent mass method and often a distinct, slightly earlier-eluting peak. Deamidation at the C-terminal amide, and truncated sequences from incomplete coupling during solid-phase synthesis, are the other categories a careful analysis will separate rather than integrate together.
What an analysis should show
Column chemistry and dimensions, mobile phase and gradient, detection wavelength or wavelengths, retention time, integrated peak area with a visible integration window, and a mass confirmation consistent with the amidated decapeptide at about 1,182 g/mol. Reported separately from chromatographic purity: net peptide content, and the declared counter-ion form. Gonadorelin carries an arginine and a histidine, so it binds counter-ion readily and the gap between purity and content is not negligible.
Naming
Gonadorelin, GnRH and LHRH all refer to the same ten-residue sequence in most catalogue usage. Analogues are a different matter entirely: substituted derivatives are separate compounds with separate masses, and a certificate for one is not evidence about another. Verify by sequence and mass, not by name.
Confirming identity independently
Public chemical databases index this compound thoroughly and by several names. Search on the sequence to resolve which entry corresponds to the material you are looking at. Our product pages carry compound identity links that run those queries directly.
How Gonadorelin is supplied here
Gonadorelin 10mg is supplied as a lyophilised powder in a sealed vial, with an independent reverse-phase HPLC analysis published on the product page alongside net peptide content, counter-ion form and vial size. Vials carry no batch or lot numbers and are matched to their published test by crimp and cap colour.
Frequently asked questions
What is the amino acid sequence of Gonadorelin?
pGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2, a ten-residue peptide with a pyroglutamate N-terminus and an amidated C-terminus.
What molecular weight should the mass spectrum show?
Approximately 1,182 g/mol for the free base. A salt form reads higher, and a free-acid C-terminus reads about 1 Da higher than the amide.
Is Gonadorelin the same as GnRH and LHRH?
In catalogue usage, yes — all three names are used for the same native decapeptide sequence. Substituted analogues sold under other names are different compounds.
Why can this peptide not be sequenced by Edman degradation?
Because the N-terminal residue is a cyclised pyroglutamate with no free alpha-amino group, and Edman chemistry requires one to begin.
Do the vials carry a lot number?
No. Vials carry no batch or lot identifiers and are matched to their published test by crimp and cap colour.
Research use only. All products supplied by Battle Born Peptides are laboratory reference materials for in-vitro research and analytical use by qualified professionals. They are not drugs, foods, dietary supplements, cosmetics or medical devices; they are not approved by the FDA or any other regulator for use in humans or animals; and they are not intended to diagnose, treat, cure, mitigate or prevent any disease, or to affect the structure or any function of the body of humans or animals. Nothing in this article is preparation, handling or dosing guidance. See our full research-use terms.