Kisspeptin 10mg

Kisspeptin 10mg: the decapeptide behind the name

The material in this 10 mg vial is registered as kisspeptin-10 (CAS 374675-21-5), a ten-residue peptide amide whose sequence reads YNWNSFGLRF-NH2 in one-letter code. Four of those ten positions are aromatic: a tyrosine at the N-terminus, a tryptophan at position 3 and phenylalanines at positions 6 and 10. Two asparagines, at positions 2 and 4, flank the tryptophan, and all ten residues are standard L-amino acids.

Next to the polar tripeptides and acidic chains elsewhere in the catalog, this is a compact and fairly hydrophobic molecule. It is the shortest of the KISS1-derived peptides and the one most often used as a reference form, but the family name alone does not specify it. Gonadorelin, another short C-terminally amidated peptide in the catalog, is identified the same way: by its terminal amide and exact residue count.

Kisspeptin 10mg specification

How Kisspeptin 10mg is analyzed

Tryptophan and tyrosine give kisspeptin-10 real absorbance near 280 nm, which sets it apart from most short research peptides. Purity is still integrated on the trace at around 214 nm, where every peptide-bonded impurity is seen, and the 280 nm channel serves as a cross-check that under-reports any impurity lacking aromatic residues. The aromatic side chains and the leucine give solid retention on C18, so the main peak elutes mid-gradient, well clear of the solvent front.

The tryptophan indole is the most oxidation-prone site in the sequence, giving +16 and +32 Da products; the two asparagines are candidates for deamidation at +1 Da; and single-residue deletions, truncated chains and residual protecting groups are the usual synthesis-related species, several of them eluting close to the main peak. An independent HPLC result specific to Kisspeptin 10mg is published with this listing; no lot label is printed, so a vial’s crimp and cap color identifies the matching run.

Research contexts for Kisspeptin 10mg

• Dual-wavelength (214 and 280 nm) HPLC purity cross-checks for aromatic peptides
• Tryptophan oxidation and asparagine deamidation profiling by LC-MS
• Concentration-response studies at KISS1R in recombinant cell-based assays
• Use as a reference peptide in analytical panels of short amidated peptides

The 10 mg vial serves chromatographers and cell-based assay groups working in vitro; using kisspeptin-10 in people or animals falls entirely outside its purpose.

Kisspeptin 10mg questions

Why does kisspeptin-10 show a signal at 280 nm?

Its tryptophan and tyrosine absorb strongly near 280 nm. Purity is nevertheless reported from the 214 nm trace, where impurities without aromatic residues are also detected.

What does a +16 Da peak mean for kisspeptin-10?

It usually indicates oxidation of the tryptophan side chain. A +1 Da shift is more likely to be deamidation at one of the two asparagines.

How many moles of peptide does 10 mg of kisspeptin-10 represent?

At about 1302.4 g/mol, 10 mg of pure free base corresponds to roughly 7.7 µmol. The real figure is lower, because the counter-ion and residual water in a lyophilized salt add mass without adding peptide.